polyclonal goat anti mouse coagulation factor vii primary antibody  (R&D Systems)

Journal: Oncotarget

Article Title: Chimeric antigen receptor-modified T Cells inhibit the growth and metastases of established tissue factor-positive tumors in NOG mice

doi: 10.18632/oncotarget.14367

Figure Lengend Snippet: ( A ) Structure diagram of TF-CAR. mlF VII, the light chain of mouse coagulation factor VII; TMD, transmembrane domain. ( B ) Western bloting analysis of TF-CAR expression in T cells. CAR-T, TF-CAR T cells; CON-T, vector-transduced T cells; T, untreated T cells. ( C ) Flow cytometry analysis of TF-CAR expression in transduced T cells. ( D ) Immunofluorescence staining analysis of TF-CAR expression in transduced T cells. The images were taken under × 400 magnification. ( E ) CAR positive ratio analysis of lentivirus infected T cells by Flow cytometry. All sample were prepared after 5 days of virus infection.

Article Snippet: The following antigens and antibodies were used for this analysis: human TF, mouse anti-human TF primary antibody (R&D Systems, Minnesota, USA) and FITC-conjugated anti-mouse IgG1 secondary antibody (eBioscience, San Diego, CA); mouse TF, PE-conjugated goat anti-mouse TF antibody (R&D Systems, Minnesota, USA); TF-CAR, polyclonal goat anti-mouse coagulation factor VII primary antibody (R&D Systems, Minnesota, USA) and PE-conjugated rabbit anti-goat IgG secondary antibody (Santa Cruz, CA, USA).

Techniques: Coagulation, Western Blot, Expressing, Plasmid Preparation, Flow Cytometry, Immunofluorescence, Staining, Infection, Virus